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Investigation the gene expression of interleukins 6 in the testis and sperm characteristics of roosters during puberty
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Reza Khostavaneh   , Mehrdad Meamar * , Javad Habibi Zad , Mustafa Muhaghegh Dolat abady , Reza Naghiha Najafabadi |
| Animal science departement , meamar@yu.ac.ir |
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Abstract: (169 Views) |
In order to investigate the changes in interleukin 6 gene expression and the changes of sperm characteristics during the maturation period of native roosters, the present study was conducted as a completely randomized design with 4 treatments (rooster age) and 6 replications. For this purpose, 24 native roosters aged 5, 6, 7, and 8 months were purchased from the center for breeding chickens and native roosters, and after slaughtering and withdrawing the visceral organs, semen samples were collected from vasdeferens and the characteristics of the semen were immediately determined. The testicle samples were also transferred to the laboratory to extract the interleukin 6 mRNA gene and check its expression changes. The results showed that the percentage of motile spermatozoa was the lowest at 5 months and did not change significantly from 6 to 8 months. The percentage of live sperm was the lowest at 5 and 6 months compared to 7 and 8 months, and at the same time, the sperm volume and sperm concentration were the lowest at 5 months and the highest at 8 months. The results of interleukin 6 gene expression also showed that this gene was more expressed at 6, 7 and 8 months than at 5 months, while the expression of this gene increased significantly at 7 months compared to 6 months, but there was not any significant difference between 7 and 8 months.
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| Keywords: Interleukin, sperm, cock, gene |
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Full-Text [PDF 612 kb]
(84 Downloads)
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Type of Study: Research Article |
Subject:
Animal physiology
Received: 2024/05/8 | Accepted: 2024/10/20 | Published: 2024/12/30
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