en موضوع 4 Animal genetics پژوهشي Research Article <span style="font-size:11pt"><span style="unicode-bidi:embed"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="font-size:12.0pt"><span style="line-height:150%"><span style="color:black">Glucose is a pervasive fuel in biology and is an energy source in most living organisms. Blood glucose level is an important physiological factor that is controlled by hormones, hormone receptors, and certain regulatory proteins. Studies of different organisms have shown that the control and regulation of glucose is very different amongst different organisms. The present study aimed to reconstruct the genes in <i>G. varius</i> and then to investigate possible genetic variations in genes involved in glucose homeostasis pathway between <i>G. gallus</i> and <i>G. varius</i> to determine the cause of blood glucose level difference in these two species. <i>GIPR, GCGR, GLP-2R, GLP-1R, and GCG</i> genes in the Gallus family were identified as genes involved in blood glucose homeostasis from various studies. From the NCBI database, the nucleotide sequences of these genes from <i>G. gallus</i> were obtained in FASTA format and blasted with tblastn tools with SRAs related to <i>G. varius</i>. Then, the nucleotide and amino acid sequences of these genes for <i>G. varius</i> were predicted and their polymorphisms with corresponding genes in <i>G. gallus</i> were identified. The results indicated a single nucleotide deletion in the <i>G. varius</i> glucagon receptor which leads to a fameshift and premature stop codon. This mutation deletes last two transmembrane domains of this receptor. The results of molecular dynamics simulations also confirmed the dramatic changes in the structure of the glucagon receptor gene.</span></span></span></span></span></span></span><br> &nbsp; Glucose homeostasis, G. gallus, G. varius, frameshift mutation, GCGR gene 33 40 http://ijls.yu.ac.ir/browse.php?a_code=A-10-46-1&slc_lang=en&sid=1 Fatemeh Dashti dashti569@gmail.com 1003194753284600192 1003194753284600192 No Shahrekord University Karim Mahnam mahnam.harim@sku.ac.ir 1003194753284600193 1003194753284600193 No Shahrekord University Mostafa Shakhsi-Niaei shakhsi-niaei.m@sku.ac.ir 1003194753284600194 1003194753284600194 Yes Shahrekord University