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A frameshift mutation (c.1021delC) in GCGR in Gallus varius may interpret its higher blood glucose level in comparison to G. gallus
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Fatemeh Dashti   , Karim Mahnam , Mostafa Shakhsi-Niaei * |
| Shahrekord University & Research institute of Biotechnology , shakhsi-niaei.m@sku.ac.ir |
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Abstract: (338 Views) |
Glucose is a pervasive fuel in biology and is an energy source in most living organisms. Blood glucose level is an important physiological factor that is controlled by hormones, hormone receptors, and certain regulatory proteins. Studies of different organisms have shown that the control and regulation of glucose is very different amongst different organisms. The present study aimed to reconstruct the genes in G. varius and then to investigate possible genetic variations in genes involved in glucose homeostasis pathway between G. gallus and G. varius to determine the cause of blood glucose level difference in these two species. GIPR, GCGR, GLP-2R, GLP-1R, and GCG genes in the Gallus family were identified as genes involved in blood glucose homeostasis from various studies. From the NCBI database, the nucleotide sequences of these genes from G. gallus were obtained in FASTA format and blasted with tblastn tools with SRAs related to G. varius. Then, the nucleotide and amino acid sequences of these genes for G. varius were predicted and their polymorphisms with corresponding genes in G. gallus were identified. The results indicated a single nucleotide deletion in the G. varius glucagon receptor which leads to a fameshift and premature stop codon. This mutation deletes last two transmembrane domains of this receptor. The results of molecular dynamics simulations also confirmed the dramatic changes in the structure of the glucagon receptor gene.
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| Keywords: Glucose homeostasis, G. gallus, G. varius, frameshift mutation, GCGR gene |
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Full-Text [PDF 856 kb]
(112 Downloads)
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Type of Study: Research Article |
Subject:
Animal genetics
Received: 2024/05/20 | Accepted: 2024/10/3 | Published: 2024/06/20
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